{"id":2417,"date":"2015-10-08T19:35:00","date_gmt":"2015-10-08T23:35:00","guid":{"rendered":"https:\/\/www.med.unc.edu\/pharm\/sondeklab\/resources\/protein-purification-analysis-protocols\/"},"modified":"2018-10-09T11:51:57","modified_gmt":"2018-10-09T15:51:57","slug":"protein-purification-analysis-protocols","status":"publish","type":"page","link":"https:\/\/www.med.unc.edu\/pharm\/sondeklab\/resources\/protein-purification-analysis-protocols\/","title":{"rendered":"Protein Purification \/ Analysis Protocols"},"content":{"rendered":"<ul>\n<li><a href=\"https:\/\/www.med.unc.edu\/pharm\/sondeklab\/wp-content\/uploads\/sites\/868\/2018\/10\/Exchange_assays_-measuring_MANT-GTP_fluorescence-_2.doc\" target=\"_self\">Exchange assays (MANT-GTP fluorescence)<\/a><\/li>\n<li><a href=\"https:\/\/www.med.unc.edu\/pharm\/sondeklab\/wp-content\/uploads\/sites\/868\/2018\/10\/Exchange_assays_-measuring_tryp_fluorescence-_2.doc\" target=\"_self\">Exchange assays (Trp fluorescence)<\/a><\/li>\n<li><a href=\"https:\/\/www.med.unc.edu\/pharm\/sondeklab\/wp-content\/uploads\/sites\/868\/2018\/10\/limited-proteolysis-protocol-v2.docx\" target=\"_self\">Limited proteolysis<\/a><\/li>\n<li><a href=\"https:\/\/www.med.unc.edu\/pharm\/sondeklab\/wp-content\/uploads\/sites\/868\/2018\/10\/SeMet_protocol.doc\" target=\"_self\">Seleno-methionine media\/culture<\/a><\/li>\n<li><a href=\"https:\/\/www.med.unc.edu\/pharm\/sondeklab\/wp-content\/uploads\/sites\/868\/2018\/10\/Silver-Stain-Protocol.doc\" target=\"_self\">Silver staining protocol<\/a><\/li>\n<li><a href=\"https:\/\/www.med.unc.edu\/pharm\/sondeklab\/wp-content\/uploads\/sites\/868\/2018\/10\/Solubility-test.doc\" target=\"_self\">Small-scale His-tagged protein solubility test<\/a><\/li>\n<li><a href=\"https:\/\/www.med.unc.edu\/pharm\/sondeklab\/wp-content\/uploads\/sites\/868\/2018\/10\/Taq_Polymerase_purification.doc\" target=\"_self\">Taq polymerase purification<\/a><\/li>\n<li><a href=\"https:\/\/www.med.unc.edu\/pharm\/sondeklab\/wp-content\/uploads\/sites\/868\/2018\/10\/TCA_precipitation_protocol.doc\" target=\"_self\">TCA precipitation protocol<\/a><\/li>\n<li><a href=\"https:\/\/www.med.unc.edu\/pharm\/sondeklab\/wp-content\/uploads\/sites\/868\/2018\/10\/purificationofSuperTEVresived12032015.docx\" target=\"_self\">TEV protease purification<\/a><\/li>\n<li><a href=\"https:\/\/www.med.unc.edu\/pharm\/sondeklab\/wp-content\/uploads\/sites\/868\/2018\/10\/Western_Blot_Protocol.doc\" target=\"_self\">Western blot protocol<\/a><\/li>\n<li><a href=\"https:\/\/www.med.unc.edu\/pharm\/sondeklab\/wp-content\/uploads\/sites\/868\/2018\/10\/ZYM-5052.doc\" target=\"_self\">ZYM media composition and preparation<\/a><\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>Exchange assays (MANT-GTP fluorescence) Exchange assays (Trp fluorescence) Limited proteolysis Seleno-methionine media\/culture Silver staining protocol Small-scale His-tagged protein solubility test Taq polymerase purification TCA precipitation protocol TEV protease purification Western blot protocol ZYM media composition and preparation<\/p>\n","protected":false},"author":22429,"featured_media":0,"parent":2403,"menu_order":3,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-2417","page","type-page","status-publish","hentry","odd"],"acf":[],"_links_to":[],"_links_to_target":[],"_links":{"self":[{"href":"https:\/\/www.med.unc.edu\/pharm\/sondeklab\/wp-json\/wp\/v2\/pages\/2417","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.med.unc.edu\/pharm\/sondeklab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.med.unc.edu\/pharm\/sondeklab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/pharm\/sondeklab\/wp-json\/wp\/v2\/users\/22429"}],"replies":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/pharm\/sondeklab\/wp-json\/wp\/v2\/comments?post=2417"}],"version-history":[{"count":0,"href":"https:\/\/www.med.unc.edu\/pharm\/sondeklab\/wp-json\/wp\/v2\/pages\/2417\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/pharm\/sondeklab\/wp-json\/wp\/v2\/pages\/2403"}],"wp:attachment":[{"href":"https:\/\/www.med.unc.edu\/pharm\/sondeklab\/wp-json\/wp\/v2\/media?parent=2417"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}