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Inverted confocal microscope with a motorized stage, resonant and galvano scanners, and a stage-top incubator with controlled temperature, CO2 and humidity levels.

Location: 5114 MEJ

Modalities

  • Z-stack Acquisition
  • Mosaic Imaging
  • FRAP
  • Deconvolution
  • Time-lapse Live Cell Multiposition Imaging

Specifications

Objectives

MagnificationNAWD (mm)CorrectionImmersionNotes
2X0.086.2Plan Apochromat Air
0.1613Super ApochromatAirAvailable upon request
10X0.310Semi ApochromatAirAvailable upon request
0.403.1Super ApochromatAir
0.750.6Super ApochromatAir
0.456.6-7.8Semi ApochromatAirAvailable upon request
1.050.8Super ApochromatSilicon Oil
1.400.13Extended Apochromat Oil
1.400.12Super CorrectionOil

Lasers

Excitation (nm)DyesType
405DAPI, Hoechst 33258, Alexa 405Diode
445CFPDiode
488FITC, Alexa 488, CY2, GFP, BodipyDiode
514YFPDiode
561Cy3, RFP, Alexa 546, Alexa 561, dsRed2Diode
594Alexa 594, mCherryDiode
640Cy5, DRAQ5Diode

Detectors

Types
4 highly-sensitive fluorescence spectral GaAsP detectors
(two multialkali PMT and two high sensitivity GaAsP PMT detectors)
1 transmission for brightfield, DIC

Software

Version
Cellsens

Filter Spectra

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