{"id":2287,"date":"2019-07-16T14:02:12","date_gmt":"2019-07-16T18:02:12","guid":{"rendered":"https:\/\/www.med.unc.edu\/mlicellcore\/?page_id=2287"},"modified":"2023-05-04T15:51:32","modified_gmt":"2023-05-04T19:51:32","slug":"research-applications","status":"publish","type":"page","link":"https:\/\/www.med.unc.edu\/mlicellcore\/research-applications\/","title":{"rendered":"Protocols and Research Applications"},"content":{"rendered":"<h4><strong>Core Protocols:<br \/>\n<\/strong><\/h4>\n<p><a href=\"https:\/\/www.med.unc.edu\/mlicellcore\/wp-content\/uploads\/sites\/1021\/2020\/03\/Cell-Count.pdf\">Cell Count<\/a><br \/>\n<a href=\"https:\/\/www.med.unc.edu\/mlicellcore\/wp-content\/uploads\/sites\/1021\/2020\/03\/Cell-Passaging-Double-Trypin.pdf\">Cell Passaging Double Trypsinization<\/a><br \/>\n<a href=\"https:\/\/www.med.unc.edu\/mlicellcore\/wp-content\/uploads\/sites\/1021\/2020\/01\/Cell-Passaging-with-ACCUTASE.pdf\">Cell Passaging with Accutase<\/a><br \/>\n<a href=\"https:\/\/www.med.unc.edu\/mlicellcore\/wp-content\/uploads\/sites\/1021\/2020\/01\/Cell-plating-guidelines-01072020lf.pdf\">Cell Plating Guidelines<\/a><br \/>\n<a href=\"https:\/\/www.med.unc.edu\/mlicellcore\/wp-content\/uploads\/sites\/1021\/2020\/03\/HPC-Collagen-IV-Coating-Inserts-WEB.pdf\">Coating Inserts- HPC-Collagen IV<\/a><br \/>\n<a href=\"https:\/\/www.med.unc.edu\/mlicellcore\/wp-content\/uploads\/sites\/1021\/2020\/01\/CoatPlates-Update-01072020lf-WEB.pdf\">Coating Plastic Plates<\/a><br \/>\n<a href=\"https:\/\/www.med.unc.edu\/mlicellcore\/wp-content\/uploads\/sites\/1021\/2020\/03\/Freeze-Thaw-Protocol-WEB.pdf\">Freeze and Thaw Protocol<\/a><br \/>\n<a href=\"https:\/\/www.med.unc.edu\/mlicellcore\/wp-content\/uploads\/sites\/1021\/2020\/03\/Maintenance-of-HBE-Cells-on-Plastic-WEB.pdf\">Maintenance of HBE Cells on Plastic<\/a><br \/>\n<a href=\"https:\/\/www.med.unc.edu\/mlicellcore\/wp-content\/uploads\/sites\/1021\/2021\/12\/Primary-HBE-Instructions-Dec-2021.pdf\">Primary HBE Instructions<\/a><br \/>\n<a href=\"https:\/\/www.med.unc.edu\/mlicellcore\/wp-content\/uploads\/sites\/1021\/2020\/03\/Well-Differentiated-Cell-cultures-WEB.pdf\">Well-Differentiated Cell Cultures<\/a><br \/>\n<a href=\"https:\/\/www.med.unc.edu\/mlicellcore\/wp-content\/uploads\/sites\/1021\/2020\/03\/BMI-Cell-Line-Instructions-2020-Final.pdf\">BMI Cell Line Instructions<\/a><\/p>\n<h4><strong>Papers Featuring Our Research:<\/strong><\/h4>\n<p><a href=\"https:\/\/www.med.unc.edu\/marsicolunginstitute\/files\/2017\/12\/Book-Chapter.pdf\">Well-Differentiated Human Airway Epithelial Cell Culture<\/a><\/p>\n<p><a href=\"https:\/\/www.med.unc.edu\/mlicellcore\/wp-content\/uploads\/sites\/1021\/2020\/04\/ajplung.90314.2008.pdf\">Novel Human Bronchial Epithelial Cell Lines for Cystic Fibrosis Research<\/a><\/p>\n<p><a href=\"https:\/\/www.med.unc.edu\/marsicolunginstitute\/files\/2017\/12\/Chapter-18.pdf\">Primary Epithelial Cell Models for Cystic Fibrosis Research<\/a><\/p>\n<p><a href=\"\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC5449492\/pdf\/rcmb.2016-0276MA.pdf\" target=\"_blank\" rel=\"noopener\">Pharmacological Rescue of Conditionally Reprogrammed CF HBE cells<\/a><\/p>\n<h4><\/h4>\n<h4><strong>Below is our training video which goes over our protocols and aseptic technique:<\/strong><\/h4>\n\n<!-- iframe plugin v.5.2 wordpress.org\/plugins\/iframe\/ -->\n<iframe loading=\"lazy\" src=\"https:\/\/uncch.hosted.panopto.com\/Panopto\/Pages\/Embed.aspx?id=af463a70-3ec1-4881-9129-ae20013f61a8&#038;autoplay=false&#038;offerviewer=true&#038;showtitle=true&#038;showbrand=true&#038;captions=true&#038;interactivity=all\" height=\"405\" width=\"720\" style=\"border: 1px solid #464646;\" 0=\"allowfullscreen\" 1=\"allow=&quot;autoplay&quot;&gt;&lt;\/iframe\" scrolling=\"yes\" class=\"iframe-class\" frameborder=\"0\"><\/iframe>\n\n<p>&nbsp;<\/p>\n<div id=\"ensembleEmbeddedContent_1ri7ggq0nEuLW1_yu6Tqyw\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Core Protocols: Cell Count Cell Passaging Double Trypsinization Cell Passaging with Accutase Cell Plating Guidelines Coating Inserts- HPC-Collagen IV Coating Plastic Plates Freeze and Thaw Protocol Maintenance of HBE Cells on Plastic Primary HBE Instructions Well-Differentiated Cell Cultures BMI Cell Line Instructions Papers Featuring Our Research: Well-Differentiated Human Airway Epithelial Cell Culture Novel Human Bronchial &hellip; <a href=\"https:\/\/www.med.unc.edu\/mlicellcore\/research-applications\/\" aria-label=\"Read more about Protocols and Research Applications\">Read more<\/a><\/p>\n","protected":false},"author":22214,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-2287","page","type-page","status-publish","hentry","odd"],"acf":[],"_links_to":[],"_links_to_target":[],"_links":{"self":[{"href":"https:\/\/www.med.unc.edu\/mlicellcore\/wp-json\/wp\/v2\/pages\/2287","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.med.unc.edu\/mlicellcore\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.med.unc.edu\/mlicellcore\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/mlicellcore\/wp-json\/wp\/v2\/users\/22214"}],"replies":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/mlicellcore\/wp-json\/wp\/v2\/comments?post=2287"}],"version-history":[{"count":50,"href":"https:\/\/www.med.unc.edu\/mlicellcore\/wp-json\/wp\/v2\/pages\/2287\/revisions"}],"predecessor-version":[{"id":3438,"href":"https:\/\/www.med.unc.edu\/mlicellcore\/wp-json\/wp\/v2\/pages\/2287\/revisions\/3438"}],"wp:attachment":[{"href":"https:\/\/www.med.unc.edu\/mlicellcore\/wp-json\/wp\/v2\/media?parent=2287"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}