{"id":554,"date":"2010-06-30T13:50:00","date_gmt":"2010-06-30T17:50:00","guid":{"rendered":"https:\/\/med.sites.unc.edu\/cfpulmcenter\/core-facilities\/molecular-biology-core\/"},"modified":"2022-10-12T15:51:09","modified_gmt":"2022-10-12T19:51:09","slug":"molecular-biology-core","status":"publish","type":"page","link":"https:\/\/www.med.unc.edu\/marsicolunginstitute\/core-facilities\/molecular-biology-core\/","title":{"rendered":"The Molecular Biology Core"},"content":{"rendered":"<p><!-- portlet_header --><\/p>\n<p><!-- portlet_above --><\/p>\n<p><!-- description --><\/p>\n<p>Director: Wanda K O&#8217;Neal, PhD, Associate Professor<\/p>\n<h2 style=\"text-align: left\"><span style=\"color: #000000\">Mission<\/span><\/h2>\n<p class=\" \" style=\"text-align: justify;color: #666666;line-height: 1.43em\"><span style=\"text-align: justify;color: #666666;line-height: 1.43em\">Molecular biology has become an indispensable component of modern biological research programs. The Molecular Biology Core was established to provide expertise, equipment, training, and reagents to serve the molecular biology needs for the Marsico Lung Institute\/ºÚÁÏÍø Cystic Fibrosis Center. The Core houses all the necessary equipment and expertise to conduct the following services:<\/span><\/p>\n<h2 style=\"text-align: justify\"><span style=\"color: #000000\"><span style=\"line-height: 22.88px\">Services<\/span><\/span><\/h2>\n<ul>\n<li style=\"text-align: justify\">The Core serves as a centralized resource for cloning and sequencing of genes.<\/li>\n<li style=\"text-align: justify\">Isolation, storage, and analysis of high quality RNA, including RNA sequencing.<\/li>\n<li>Single-cell library preparation with 10X Chromium technology.<\/li>\n<li style=\"text-align: justify\">RNA in situ hybridization.<\/li>\n<li style=\"text-align: justify\">Generation of transgenic mouse models.<\/li>\n<li style=\"text-align: justify\">Development of methods for si\/shRNA knockdown in airway cell models.<\/li>\n<li style=\"text-align: justify\">Use of CRISR\/cas9 mediated gene editing for airway models.<\/li>\n<li style=\"text-align: justify\">Immunohistochemistry and western blotting.<\/li>\n<li style=\"text-align: justify\">Training in molecular biology techniques.<\/li>\n<\/ul>\n<h2 style=\"color: #4d4d4d;line-height: 1.3em\"><span style=\"color: #4d4d4d;line-height: 1.3em\">Contact Information<\/span><\/h2>\n<address style=\"text-align: left\">125 Mason Farm Road<br \/>\nCampus Box #7248<br \/>\nThe University of North Carolina at Chapel Hill<br \/>\nChapel Hill, NC 27599<br \/>\n(919) 962 9866<br \/>\nFax: (919) 966-5178<br \/>\nE-mail: <a href=\"mailto:woneal@email.unc.edu\">woneal@email.unc.edu<\/a>&nbsp;<\/p>\n<p><strong>Contacts:<\/strong><br \/>\nRodney Gilmore, Research Specialist: <a href=\"mailto:rodney_gilmore@med.unc.edu\">rodney_gilmore@med.unc.edu<\/a><br \/>\nLisa Morton, Research Specialist:\u00a0<a href=\"mailto:lisa_morton@med.unc.edu\">lisa_morton@med.unc.edu<\/a><\/p>\n<\/address>\n","protected":false},"excerpt":{"rendered":"<p>Director: Wanda K O&#8217;Neal, PhD, Associate Professor Mission Molecular biology has become an indispensable component of modern biological research programs. The Molecular Biology Core was established to provide expertise, equipment, training, and reagents to serve the molecular biology needs for the Marsico Lung Institute\/ºÚÁÏÍø Cystic Fibrosis Center. The Core houses all the necessary equipment and &hellip; <a href=\"https:\/\/www.med.unc.edu\/marsicolunginstitute\/core-facilities\/molecular-biology-core\/\" aria-label=\"Read more about The Molecular Biology Core\">Read more<\/a><\/p>\n","protected":false},"author":76119,"featured_media":0,"parent":62,"menu_order":1,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-554","page","type-page","status-publish","hentry","odd"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v23.9 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>The Molecular Biology Core | Marsico Lung Institute<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.med.unc.edu\/marsicolunginstitute\/core-facilities\/molecular-biology-core\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"The Molecular Biology Core | Marsico Lung Institute\" \/>\n<meta property=\"og:description\" content=\"Director: Wanda K O&#8217;Neal, PhD, Associate Professor Mission Molecular biology has become an indispensable component of modern biological research programs. 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