{"id":2535,"date":"2020-09-27T18:12:39","date_gmt":"2020-09-27T22:12:39","guid":{"rendered":"https:\/\/www.med.unc.edu\/cryo-em\/?page_id=2535"},"modified":"2022-12-21T10:54:20","modified_gmt":"2022-12-21T15:54:20","slug":"protocols","status":"publish","type":"page","link":"https:\/\/www.med.unc.edu\/cryo-em\/protocols\/","title":{"rendered":"Protocols"},"content":{"rendered":"<h3><strong>Preparing Cryo-Grids With The Vitrobot Mark IV.<\/strong><\/h3>\n<p>We use this protocol to prepare cryo-grids of purified protein completes, nano-particles, and virus-like particles. \u00a0If you would like to learn how to prepare cryo-grids or need assistance operating the Vitrobot, please contact us.<\/p>\n<p><a href=\"https:\/\/www.med.unc.edu\/cryo-em\/wp-content\/uploads\/sites\/1040\/2021\/10\/Vitrobot_20211015.pdf\">Vitrobot Protocol 20211015<\/a><\/p>\n<h3><strong>Operating the Talos Arctica using SerialEM.<\/strong><\/h3>\n<p>We use this protocol to operate the Talos Arctica for single-particle cryoEM. \u00a0We routinely use the microscope for screening and data collection. \u00a0If you would like to learn how to operate the microscope, please contact us directly.<\/p>\n<p><a href=\"https:\/\/www.med.unc.edu\/cryo-em\/wp-content\/uploads\/sites\/1040\/2022\/12\/20221221_TalosArctica_Protocol_V2docx.pdf\">20210804_Talos_Arctica<\/a><\/p>\n<h3><strong>Preparing Graphene Oxide TEM Grids.<\/strong><\/h3>\n<p>We use this protocol to transfer a single mono-layer of graphene oxide (GO) onto Quantofil TEM grids for cryoEM applications. \u00a0This includes single-particle cryoEM of purified protein complexes as well as liposomes or nanoparticles. \u00a0If you would like to learn how to prepare your own GO TEM grids or would like to like the Core to prepare some GO TEM grids for you, please contact us directly.<\/p>\n<p><a href=\"https:\/\/www.med.unc.edu\/cryo-em\/wp-content\/uploads\/sites\/1040\/2021\/07\/GO_Procedure_V4.pdf\">GO_Procedure_V4<\/a><\/p>\n<h3><strong>Convert Quantifoil or CFlat TEM Grids to UltrAufoil TEM Grids.<\/strong><\/h3>\n<p>We use this protocol to convert Quantifoil or CFlat TEM grids to UltrAufoil TEM grids. \u00a0Grids are sputter coated with gold and plasma cleaned to remove carbon film. \u00a0If you have any questions about this protocol please contact us.<\/p>\n<p><a href=\"https:\/\/www.med.unc.edu\/cryo-em\/wp-content\/uploads\/sites\/1040\/2022\/04\/QF2AU.pdf\">QF2AU<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Preparing Cryo-Grids With The Vitrobot Mark IV. We use this protocol to prepare cryo-grids of purified protein completes, nano-particles, and virus-like particles. \u00a0If you would like to learn how to prepare cryo-grids or need assistance operating the Vitrobot, please contact us. Vitrobot Protocol 20211015 Operating the Talos Arctica using SerialEM. We use this protocol to &hellip; <a href=\"https:\/\/www.med.unc.edu\/cryo-em\/protocols\/\" aria-label=\"Read more about Protocols\">Read more<\/a><\/p>\n","protected":false},"author":93771,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-2535","page","type-page","status-publish","hentry","odd"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v23.9 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Protocols - Cryo EM Core<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.med.unc.edu\/cryo-em\/protocols\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Protocols - Cryo EM Core\" \/>\n<meta property=\"og:description\" content=\"Preparing Cryo-Grids With The Vitrobot Mark IV. We use this protocol to prepare cryo-grids of purified protein completes, nano-particles, and virus-like particles. \u00a0If you would like to learn how to prepare cryo-grids or need assistance operating the Vitrobot, please contact us. Vitrobot Protocol 20211015 Operating the Talos Arctica using SerialEM. We use this protocol to &hellip; Read more\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.med.unc.edu\/cryo-em\/protocols\/\" \/>\n<meta property=\"og:site_name\" content=\"Cryo EM Core\" \/>\n<meta property=\"article:modified_time\" content=\"2022-12-21T15:54:20+00:00\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/www.med.unc.edu\/cryo-em\/protocols\/\",\"url\":\"https:\/\/www.med.unc.edu\/cryo-em\/protocols\/\",\"name\":\"Protocols - Cryo EM Core\",\"isPartOf\":{\"@id\":\"https:\/\/www.med.unc.edu\/cryo-em\/#website\"},\"datePublished\":\"2020-09-27T22:12:39+00:00\",\"dateModified\":\"2022-12-21T15:54:20+00:00\",\"breadcrumb\":{\"@id\":\"https:\/\/www.med.unc.edu\/cryo-em\/protocols\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/www.med.unc.edu\/cryo-em\/protocols\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/www.med.unc.edu\/cryo-em\/protocols\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\/\/www.med.unc.edu\/cryo-em\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Protocols\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/www.med.unc.edu\/cryo-em\/#website\",\"url\":\"https:\/\/www.med.unc.edu\/cryo-em\/\",\"name\":\"Cryo EM Core\",\"description\":\"Center for Structural Biology\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/www.med.unc.edu\/cryo-em\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Protocols - Cryo EM Core","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.med.unc.edu\/cryo-em\/protocols\/","og_locale":"en_US","og_type":"article","og_title":"Protocols - Cryo EM Core","og_description":"Preparing Cryo-Grids With The Vitrobot Mark IV. We use this protocol to prepare cryo-grids of purified protein completes, nano-particles, and virus-like particles. \u00a0If you would like to learn how to prepare cryo-grids or need assistance operating the Vitrobot, please contact us. Vitrobot Protocol 20211015 Operating the Talos Arctica using SerialEM. We use this protocol to &hellip; Read more","og_url":"https:\/\/www.med.unc.edu\/cryo-em\/protocols\/","og_site_name":"Cryo EM Core","article_modified_time":"2022-12-21T15:54:20+00:00","twitter_card":"summary_large_image","twitter_misc":{"Est. reading time":"1 minute"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/www.med.unc.edu\/cryo-em\/protocols\/","url":"https:\/\/www.med.unc.edu\/cryo-em\/protocols\/","name":"Protocols - Cryo EM Core","isPartOf":{"@id":"https:\/\/www.med.unc.edu\/cryo-em\/#website"},"datePublished":"2020-09-27T22:12:39+00:00","dateModified":"2022-12-21T15:54:20+00:00","breadcrumb":{"@id":"https:\/\/www.med.unc.edu\/cryo-em\/protocols\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/www.med.unc.edu\/cryo-em\/protocols\/"]}]},{"@type":"BreadcrumbList","@id":"https:\/\/www.med.unc.edu\/cryo-em\/protocols\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/www.med.unc.edu\/cryo-em\/"},{"@type":"ListItem","position":2,"name":"Protocols"}]},{"@type":"WebSite","@id":"https:\/\/www.med.unc.edu\/cryo-em\/#website","url":"https:\/\/www.med.unc.edu\/cryo-em\/","name":"Cryo EM Core","description":"Center for Structural Biology","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/www.med.unc.edu\/cryo-em\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"}]}},"_links_to":[],"_links_to_target":[],"_links":{"self":[{"href":"https:\/\/www.med.unc.edu\/cryo-em\/wp-json\/wp\/v2\/pages\/2535","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.med.unc.edu\/cryo-em\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.med.unc.edu\/cryo-em\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/cryo-em\/wp-json\/wp\/v2\/users\/93771"}],"replies":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/cryo-em\/wp-json\/wp\/v2\/comments?post=2535"}],"version-history":[{"count":15,"href":"https:\/\/www.med.unc.edu\/cryo-em\/wp-json\/wp\/v2\/pages\/2535\/revisions"}],"predecessor-version":[{"id":3001,"href":"https:\/\/www.med.unc.edu\/cryo-em\/wp-json\/wp\/v2\/pages\/2535\/revisions\/3001"}],"wp:attachment":[{"href":"https:\/\/www.med.unc.edu\/cryo-em\/wp-json\/wp\/v2\/media?parent=2535"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}