{"id":3737,"date":"2020-05-01T09:13:40","date_gmt":"2020-05-01T13:13:40","guid":{"rendered":"https:\/\/www.med.unc.edu\/corefacilities\/?p=3737"},"modified":"2020-05-01T09:16:08","modified_gmt":"2020-05-01T13:16:08","slug":"mass-spectrometry-based-lipidomics-methods-now-available-in-the-chemistry-mass-spectrometry-core","status":"publish","type":"post","link":"https:\/\/www.med.unc.edu\/corefacilities\/mass-spectrometry-based-lipidomics-methods-now-available-in-the-chemistry-mass-spectrometry-core\/","title":{"rendered":"Mass spectrometry-based lipidomics methods now available in the Chemistry Mass Spectrometry Core"},"content":{"rendered":"<p>The Department of Chemistry Mass Spectrometry Core Laboratory is excited to offer mass spectrometry based lipidomics to the ºÚÁÏÍø Community.\u00a0 With Method Development Grant funding from the Core Facilities Advocacy Committee (CFAC), we have developed mass spectrometry based methods for the extraction, chromatographic separation, detection and data processing of lipids from biological matrices.\u00a0 Our methods target a diverse array of lipid compound classes to allow for comprehensive sample profiling. We can provide lipid identification and\/or relative quantitation methods for your experimental needs.<br \/>\nData are generated via LC-MS methods with our Waters Acquity LC system coupled to a Q Exactive HFX mass spectrometer.\u00a0 The system provides high resolution and accurate mass detection of lipid compounds.\u00a0 We utilize LipidSearch\u2122 software for lipid identification, spectral matching, and relative quantitation.<\/p>\n<figure id=\"attachment_3738\" class=\"thumbnail wp-caption alignnone\" style=\"width: 491px\"><a href=\"https:\/\/www.med.unc.edu\/corefacilities\/wp-content\/uploads\/sites\/943\/2020\/05\/ChemMS-Lipidomics-dataset-image.png\"><img loading=\"lazy\" decoding=\"async\" class=\" wp-image-3738\" src=\"https:\/\/www.med.unc.edu\/corefacilities\/wp-content\/uploads\/sites\/943\/2020\/05\/ChemMS-Lipidomics-dataset-image-300x178.png\" alt=\"Figure Caption: Some representative data from a study within the MS Core Laboratory. Our visualization of the data can be customized to our clients in order to best communicate the experimental findings\" width=\"481\" height=\"285\" srcset=\"https:\/\/www.med.unc.edu\/corefacilities\/wp-content\/uploads\/sites\/943\/2020\/05\/ChemMS-Lipidomics-dataset-image-300x178.png 300w, https:\/\/www.med.unc.edu\/corefacilities\/wp-content\/uploads\/sites\/943\/2020\/05\/ChemMS-Lipidomics-dataset-image-1024x608.png 1024w, https:\/\/www.med.unc.edu\/corefacilities\/wp-content\/uploads\/sites\/943\/2020\/05\/ChemMS-Lipidomics-dataset-image-768x456.png 768w, https:\/\/www.med.unc.edu\/corefacilities\/wp-content\/uploads\/sites\/943\/2020\/05\/ChemMS-Lipidomics-dataset-image-600x356.png 600w, https:\/\/www.med.unc.edu\/corefacilities\/wp-content\/uploads\/sites\/943\/2020\/05\/ChemMS-Lipidomics-dataset-image.png 1200w\" sizes=\"auto, (max-width: 481px) 100vw, 481px\" \/><\/a><figcaption class=\"caption wp-caption-text\"><span style=\"font-size: 12px\">Figure Caption: Some representative data from a study within the MS Core Laboratory.\u00a0 Visualization of the data can be customized to the client in order to best communicate the experimental findings<\/span><\/figcaption><\/figure>\n<p>The core also offers other mass spectrometry platforms to allow for the analysis of volatile lipids and absolute quantification of lipid species, and is equipped with a BSL 2 laboratory for biological hazard sample handling.<\/p>\n<p>The ºÚÁÏÍø Department of Chemistry Chemical Research Instrumentation Teaching and Core Laboratories, CRITCL, Mass Spectrometry Core laboratory specializes in small molecule analysis. Our services include, but are not limited to:<\/p>\n<ul>\n<li>\u00a0 complex mixture analysis using chromatography MS<\/li>\n<li>\u00a0 lipidomics (profiling and quantitation)<\/li>\n<li>\u00a0 metabolomics<\/li>\n<li>\u00a0 molecular formula confirmation using accurate mass<\/li>\n<li>\u00a0 small molecule quantitation using chromatography (gas or liquid) coupled to mass spectrometry (MS).<\/li>\n<li>\u00a0 structural elucidation using MS\/MS and MSn<\/li>\n<\/ul>\n<p>The laboratory provides individualized user training for undergraduate, graduate, and post-doctoral scholars of the Department of Chemistry and ºÚÁÏÍø campus. The laboratory aims to provide the highest quality mass spectrometric services possible to its users and clients.<\/p>\n<p>Visit the <a href=\"https:\/\/chem.unc.edu\/critcl-main\/criticl-mass-main\/\">Department of Chemistry Mass Spectrometry Core website<\/a> for more information<\/p>\n<p><a href=\"https:\/\/www.med.unc.edu\/corefacilities\/wp-content\/uploads\/sites\/943\/2020\/05\/ChemMS-Lipidomics-Flyer-2020_v3.pdf\">Lipidomics services brochure<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>The Department of Chemistry Mass Spectrometry Core Laboratory is excited to offer mass spectrometry based lipidomics to the ºÚÁÏÍø Community.\u00a0 With Method Development Grant funding from the Core Facilities Advocacy Committee (CFAC), we have developed mass spectrometry based methods for the extraction, chromatographic separation, detection and data processing of lipids from biological matrices.\u00a0 Our methods &hellip; <a href=\"https:\/\/www.med.unc.edu\/corefacilities\/mass-spectrometry-based-lipidomics-methods-now-available-in-the-chemistry-mass-spectrometry-core\/\" aria-label=\"Read more about Mass spectrometry-based lipidomics methods now available in the Chemistry Mass Spectrometry Core\">Read more<\/a><\/p>\n","protected":false},"author":11566,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"categories":[6],"tags":[],"class_list":["post-3737","post","type-post","status-publish","format-standard","hentry","category-spotlight","odd"],"acf":[],"featured_image":false,"featured_image_medium":false,"featured_image_medium_large":false,"featured_image_large":false,"featured_image_thumbnail":false,"featured_image_alt":false,"category_details":[{"name":"Spotlight","link":"https:\/\/www.med.unc.edu\/corefacilities\/category\/spotlight\/"}],"tag_details":[],"_links_to":[],"_links_to_target":[],"_links":{"self":[{"href":"https:\/\/www.med.unc.edu\/corefacilities\/wp-json\/wp\/v2\/posts\/3737","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.med.unc.edu\/corefacilities\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.med.unc.edu\/corefacilities\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/corefacilities\/wp-json\/wp\/v2\/users\/11566"}],"replies":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/corefacilities\/wp-json\/wp\/v2\/comments?post=3737"}],"version-history":[{"count":4,"href":"https:\/\/www.med.unc.edu\/corefacilities\/wp-json\/wp\/v2\/posts\/3737\/revisions"}],"predecessor-version":[{"id":3743,"href":"https:\/\/www.med.unc.edu\/corefacilities\/wp-json\/wp\/v2\/posts\/3737\/revisions\/3743"}],"wp:attachment":[{"href":"https:\/\/www.med.unc.edu\/corefacilities\/wp-json\/wp\/v2\/media?parent=3737"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.med.unc.edu\/corefacilities\/wp-json\/wp\/v2\/categories?post=3737"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.med.unc.edu\/corefacilities\/wp-json\/wp\/v2\/tags?post=3737"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}