{"id":2408,"date":"2018-10-01T17:25:00","date_gmt":"2018-10-01T21:25:00","guid":{"rendered":"https:\/\/www.med.unc.edu\/corefacilities\/advanced-analytics-core\/"},"modified":"2018-12-18T12:42:46","modified_gmt":"2018-12-18T17:42:46","slug":"advanced-analytics-core","status":"publish","type":"post","link":"https:\/\/www.med.unc.edu\/corefacilities\/advanced-analytics-core\/","title":{"rendered":"Advanced Analytics Core"},"content":{"rendered":"<p class=\"lead\">The AA Core is excited to announce single-cell Western blot service using the Protein Simple Milo! The Milo platform measures expression of multiple proteins across 1,000+ single cells in just 4-6 hours.<\/p>\n<div class=\"image-section\">\n<figure class=\"thumbnail wp-caption alignright\"><img loading=\"lazy\" decoding=\"async\" class=\"size-medium\" src=\"https:\/\/www.med.unc.edu\/corefacilities\/wp-content\/uploads\/sites\/943\/2018\/12\/advanced-analytics-core-image2.png\" alt=\"image2\" width=\"300\" height=\"200\" \/><figcaption class=\"caption wp-caption-text\"><\/figcaption><\/figure>\n<\/div>\n<div>\n<p>The CGIBD\u2019s Advanced Analytics (AA) Core provides high-quality, quantitative solutions for studying proteins and nucleic acids at the bulk or single-cell level. Our service offerings include:<\/p>\n<ul>\n<li>Immunoassays &amp; plate reading (ELISA &amp; <a href=\"https:\/\/www.luminexcorp.com\/xmap-technology\/\">bead-based multiplex<\/a>)<\/li>\n<li>High-throughput qPCR (<a href=\"https:\/\/www.fluidigm.com\/products\/biomark-hd-system\">Fluidigm BioMark HD<\/a>)<\/li>\n<li>Single-cell Genomics (<a href=\"https:\/\/www.fluidigm.com\/products\/c1-system\">Fluidigm C1<\/a>, droplet-based RNASeq)<\/li>\n<li>Single-cell Protein Detection &amp; Quantification (<a href=\"https:\/\/www.proteinsimple.com\/milo.html\">Protein Simple Milo<\/a>)<\/li>\n<\/ul>\n<p>The AA Core also provides ancillary FACS isolation, sequencing, and data analysis services as part of our \u201cturn-key\u201d solutions to your genomics and proteomics needs. Feel free to contact <a href=\"mailto:carlton_anderson@med.unc.edu\">Carlton Anderson<\/a> (Core Manager) or check out the <a href=\"https:\/\/www.med.unc.edu\/cgibd\/cores\/advanced-analytics\/\">CGIBD Core Webpage<\/a> for more information regarding our technologies, instruments, and workflows.<\/p>\n<p><b>HIGHLIGHTS:<\/b> <b><\/b><\/p>\n<p>The AA Core is excited to announce single-cell Western blot service using the Protein Simple Milo! The Milo platform measures expression of multiple proteins across 1,000+ single cells in just 4-6 hours. Milo captures cells on a novel \u201cscWest chip&#8221; (Fig. 1), performs SDS-PAGE, and immobilizes proteins prior to antibody probing and fluorescence imaging. Loaded chips can be stored, stripped, and re-probed multiple times within a 9-month period. Milo is a powerful tool both alone and in combination with the Core\u2019s other single cell genomics services (Fig. 2). More details regarding this promising new technology can be found on the <a href=\"https:\/\/www.med.unc.edu\/cgibd\/cores\/advanced-analytics\/single-cell-protein-detection-protein-simple-milo\/\">Core Webpage<\/a>.<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.med.unc.edu\/corefacilities\/wp-content\/uploads\/sites\/943\/2018\/12\/export.png\" alt=\"\" width=\"498\" height=\"194\" \/><\/p>\n<p><b>Figure 1. <\/b>A Protein Simple Milo scWest chip. An example of a typical fluorescence readout from two probed proteins is shown. Image Source: <a href=\"https:\/\/www.proteinsimple.com\/milo.html\">https:\/\/www.proteinsimple.com\/milo.html<\/a>.<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.med.unc.edu\/corefacilities\/wp-content\/uploads\/sites\/943\/2018\/12\/export-1.png\" alt=\"\" width=\"608\" height=\"360\" \/><\/p>\n<p><b>Figure 2.<\/b> Overview of simultaneous scRNA-Seq and scWest validation using the Protein Simple Milo platform. Image Source: <a href=\"https:\/\/www.proteinsimple.com\/milo.html\">https:\/\/www.proteinsimple.com\/milo.html<\/a>.<\/p>\n<p>The AA Core\u2019s mission is to support research into the etiology and pathogenesis of disease by providing a range of full-service, cost-effective immunoassay, genomics, and gene expression assays to the scientific community. To set up an expert consultation or begin a project with the AA Core, please contact the <a href=\"mailto:carlton_anderson@med.unc.edu\">Core Manager<\/a>.<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p><!-- description --> <\/p>\n<p class='lead'>The AA Core is excited to announce single-cell Western blot service using the Protein Simple Milo! The Milo platform measures expression of multiple proteins across 1,000+ single cells in just 4-6 hours.<\/p>\n","protected":false},"author":11566,"featured_media":2409,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"categories":[6],"tags":[3],"class_list":["post-2408","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-spotlight","tag-corespotlightstory","odd"],"acf":[],"featured_image":"https:\/\/www.med.unc.edu\/corefacilities\/wp-content\/uploads\/sites\/943\/2018\/12\/advanced-analytics-core-image2.png","featured_image_medium":"https:\/\/www.med.unc.edu\/corefacilities\/wp-content\/uploads\/sites\/943\/2018\/12\/advanced-analytics-core-image2-300x117.png","featured_image_medium_large":"https:\/\/www.med.unc.edu\/corefacilities\/wp-content\/uploads\/sites\/943\/2018\/12\/advanced-analytics-core-image2-768x300.png","featured_image_large":"https:\/\/www.med.unc.edu\/corefacilities\/wp-content\/uploads\/sites\/943\/2018\/12\/advanced-analytics-core-image2-1024x400.png","featured_image_thumbnail":"https:\/\/www.med.unc.edu\/corefacilities\/wp-content\/uploads\/sites\/943\/2018\/12\/advanced-analytics-core-image2-150x150.png","featured_image_alt":"","category_details":[{"name":"Spotlight","link":"https:\/\/www.med.unc.edu\/corefacilities\/category\/spotlight\/"}],"tag_details":[{"name":"CoreSpotlightStory","link":"https:\/\/www.med.unc.edu\/corefacilities\/tag\/corespotlightstory\/"}],"_links_to":[],"_links_to_target":[],"_links":{"self":[{"href":"https:\/\/www.med.unc.edu\/corefacilities\/wp-json\/wp\/v2\/posts\/2408","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.med.unc.edu\/corefacilities\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.med.unc.edu\/corefacilities\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/corefacilities\/wp-json\/wp\/v2\/users\/11566"}],"replies":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/corefacilities\/wp-json\/wp\/v2\/comments?post=2408"}],"version-history":[{"count":4,"href":"https:\/\/www.med.unc.edu\/corefacilities\/wp-json\/wp\/v2\/posts\/2408\/revisions"}],"predecessor-version":[{"id":2761,"href":"https:\/\/www.med.unc.edu\/corefacilities\/wp-json\/wp\/v2\/posts\/2408\/revisions\/2761"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/corefacilities\/wp-json\/wp\/v2\/media\/2409"}],"wp:attachment":[{"href":"https:\/\/www.med.unc.edu\/corefacilities\/wp-json\/wp\/v2\/media?parent=2408"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.med.unc.edu\/corefacilities\/wp-json\/wp\/v2\/categories?post=2408"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.med.unc.edu\/corefacilities\/wp-json\/wp\/v2\/tags?post=2408"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}