{"id":5162,"date":"2023-07-03T15:49:04","date_gmt":"2023-07-03T19:49:04","guid":{"rendered":"https:\/\/www.med.unc.edu\/corefacilities\/?page_id=5162"},"modified":"2024-07-26T10:41:05","modified_gmt":"2024-07-26T14:41:05","slug":"drug-development","status":"publish","type":"page","link":"https:\/\/www.med.unc.edu\/corefacilities\/find-a-core-facility\/drug-development\/","title":{"rendered":"Drug Development"},"content":{"rendered":"<div class=\"row  oscitas-bootstrap-container\">\n<div id=\"toc_container\" class=\"toc_wrap_left no_bullets\"><p class=\"toc_title\">Contents<\/p><ul class=\"toc_list\"><li><a href=\"#Analytical_Services\"><span class=\"toc_number toc_depth_1\">1<\/span> Analytical Services<\/a><ul><li><a href=\"#Advanced_Translational_Pharmacology_and_Analytical_Chemistry_ATPAC\"><span class=\"toc_number toc_depth_2\">1.1<\/span> Advanced Translational Pharmacology and Analytical Chemistry (ATPAC)<\/a><\/li><li><a href=\"#黑料网_CFAR_Clinical_Pharmacology_Analytical_Chemistry\"><span class=\"toc_number toc_depth_2\">1.2<\/span> 黑料网 CFAR Clinical Pharmacology &amp; Analytical Chemistry<\/a><\/li><\/ul><\/li><li><a href=\"#Drug_Screening\"><span class=\"toc_number toc_depth_1\">2<\/span> Drug Screening<\/a><ul><li><a href=\"#Organ_Injury_Biomarker_Core\"><span class=\"toc_number toc_depth_2\">2.1<\/span> Organ Injury Biomarker Core<\/a><\/li><li><a href=\"#Screening_Live_Cancer_Explants_SLICE_Core\"><span class=\"toc_number toc_depth_2\">2.2<\/span> Screening Live Cancer Explants (SLICE) Core<\/a><\/li><\/ul><\/li><li><a href=\"#Nanomaterials\"><span class=\"toc_number toc_depth_1\">3<\/span> Nanomaterials<\/a><ul><li><a href=\"#Nanomedicines_Characterization_Core_Facility_NCore\"><span class=\"toc_number toc_depth_2\">3.1<\/span> Nanomedicines Characterization Core Facility (NCore)<\/a><\/li><\/ul><\/li><li><a href=\"#Genetic_Screening\"><span class=\"toc_number toc_depth_1\">4<\/span> Genetic Screening<\/a><ul><li><a href=\"#黑料网_CRISPR_Core\"><span class=\"toc_number toc_depth_2\">4.1<\/span> 黑料网 CRISPR Core<\/a><\/li><\/ul><\/li><\/ul><\/div>\n\n<\/div>\n<p>&nbsp;<\/p>\n<h2><span id=\"Analytical_Services\"><span style=\"color: #345644\">Analytical Services<\/span><\/span><\/h2>\n<h3><span id=\"Advanced_Translational_Pharmacology_and_Analytical_Chemistry_ATPAC\">Advanced Translational Pharmacology and Analytical Chemistry (ATPAC)<\/span><\/h3>\n<p>The <a href=\"https:\/\/pharmacy.unc.edu\/research\/faculty-labs\/atpac\/\">Advanced Translational Pharmacology and Analytical Chemistry (ATPAC)<\/a> core lab consists of the Analytical Chemistry and Pharmacology Core (ACPC) lab and the Translational Oncology and Nanoparticle Drug Development Initiative (TONDDI) lab<\/p>\n<p>The ATPAC core lab uses analytical chemistry and pharmacologic infrastructure to support the translational development of drugs, anticancer agents, carrier-mediated agents and biologics. The lab provides expertise in translational drug development, analytical chemistry, pharmacology [pharmacokinetic (PK) and pharmacodynamic (PD); efficacy and toxicity], and biomarker studies.<\/p>\n<p><strong>Research applications:<\/strong>\u00a0 Services include LC-MS assay development, validation and analysis of samples to quantify a wide variety of drugs, ranging from small molecule therapeutics to complex drugs such as monoclonal antibodies, drug conjugates and polymers. The lab supports formulation development and CMC studies, assistance in PK\/PD study design, LC-MS\/MS or ICP-MS assay development, quantification of drugs from biological samples, PK\/PD analysis and report generation.<\/p>\n<p><strong>Instrumentation available:<\/strong>\u00a0 Thermo TSQ Quantum Ultra triple quadrupole mass spectrometers with Shimadzu LC-20AD series HPLC, Thermo LTQ Orbitrap Discovery mass spectrometer with Shimadzu LC-20AD series HPLC, Agilent 7700 inductively-coupled plasma mass spectrometer (ICP-MS), Shimadzu 20 series HPLC with fluorescence detection.<\/p>\n<p><strong>Resources:<\/strong>\u00a0Full-service sample analysis and assay development.\u00a0 Consultation on PK\/PD study design and execution.\u00a0 Data interpretation and preparation of manuscripts.<\/p>\n<p><strong>Eligible users:<\/strong>\u00a0 Researchers at 黑料网-CH, other academic institutions, industry\/commercial clients.<\/p>\n<h3><span id=\"黑料网_CFAR_Clinical_Pharmacology_Analytical_Chemistry\"><span class=\"largeFont\">黑料网 CFAR Clinical Pharmacology &amp; Analytical Chemistry<\/span><\/span><\/h3>\n<p><a title=\"\" href=\"http:\/\/unccfar.org\/portfolio\/pharmacology-analytical-chemistry\/\" target=\"_blank\" rel=\"noopener noreferrer\"><span class=\"largeFont\">黑料网 CFAR Clinical Pharmacology &amp; Analytical Chemistry<\/span><\/a> The goal of the 黑料网 Center For Aids Research (CFAR) CPAC core is to provide the research community with the expertise and resources in study design, analytical chemistry, and data analyses to support innovative animal and human clinical pharmacology investigations.<br \/>\nAll analyses are conducted in a CLIA-certified, state of the art analytical laboratory that includes high performance liquid chromatography with ultraviolet and mass spectroscopy detection.<br \/>\nServices include: Pharmacokinetic-pharmacodynamic analysis; development and validation of small molecule methods using LC\/UV, LC\/MS and LC\/MS\/MS in a wide variety of animal and human matrices; grants and clinical trials consultation and research support. We will provide you with advice on experimental design and the best methods to accomplish your objectives.<\/p>\n<h2><span id=\"Drug_Screening\">Drug Screening<\/span><\/h2>\n<h3><span id=\"Organ_Injury_Biomarker_Core\">Organ Injury Biomarker Core<\/span><\/h3>\n<p>The <a href=\"https:\/\/pharmacy.unc.edu\/research-programs\/\">Organ Injury Biomarker Core<\/a> can discover, validate, and quantify biomarkers that enable early detection and mechanistic insight of hepatic, renal, and cardiac injury.<\/p>\n<h3><span id=\"Screening_Live_Cancer_Explants_SLICE_Core\">Screening Live Cancer Explants (SLICE) Core<\/span><\/h3>\n<p>The &#8220;<a href=\"https:\/\/uncch.ilab.agilent.com\/service_center\/show_external\/6242?name=slice-core\">Screening Live Cancer Explants&#8221; (SLiCE) Core Facility<\/a> offers services to test your therapeutics on live, uncultured patient tumor tissue resected at 黑料网 Hospitals.<\/p>\n<h2><span id=\"Nanomaterials\">Nanomaterials<\/span><\/h2>\n<h3><span id=\"Nanomedicines_Characterization_Core_Facility_NCore\"><span class=\"largeFont\"><span class=\"largefont\">Nanomedicines Characterization Core Facility (NCore)<\/span><\/span><\/span><\/h3>\n<p>The <span class=\"largeFont\"><a href=\"http:\/\/ncore.web.unc.edu\/\" target=\"_blank\" rel=\"noopener noreferrer\"><span class=\"largefont\">Nanomedicines Characterization Core Facility (NCore)<\/span><\/a><\/span> provides comprehensive characterization of novel nanomedicines and nanomaterials. Services include physicochemical assays (ICP-MS, Nanosight, Capillary Electrophoresis, WES Simple Western Blotting, HPLC, FPLC, Thermogravimetric Analysis, GPC, Malvern Zetasizer, and more). Exosome isolation and characterization including size, size distribution, concentration, charge, markers expression, particle labeling. The Nanomedicines Characterization Core Facility (NCore) grants researchers an opportunity for in-depth characterization of nanomaterials and carrier-mediated agents (CMA) including: chemical composition, number average molar mass Mn, weight average molar mass Mw, polydispersity, molecular weight distribution, size, gyration radius, size distribution, shape, surface charge, count number of nanoparticles, solubility (second virial coefficient), thermal\/oxidative stabilities, estimation of product lifetime, isotope analysis, analytical evaluation of nanoparticle encapsulated and released drug or agent stability and released rates, degree and rate of recognition of nanoparticles by mononuclear phagocyte system (MPS), UV\/Vis spectroscopy, UV-temperature correlation, etc.<\/p>\n<p><b>Research applications<\/b><b>:<\/b> Physicochemical characterization (PC) of nanomaterials offers a comprehensive body of information that can be used to predict behavior of the nanomaterials in biological environments both in vitro and in vivo. This information is essential in order to exercise tight control over inter-batch variations and maintain reproducibility of nanomaterials critical for their applications.<\/p>\n<p>N-Core offers PC of various classes of nanomaterials such as:<\/p>\n<ul>\n<li>Polymer conjugates<\/li>\n<li>Polymeric micelles<\/li>\n<li>Liposomes<\/li>\n<li>Nanogels<\/li>\n<li>Polyion complexes of small drugs and biomacromolecules (proteins, DNA, and RNA)<\/li>\n<li>Inorganic\/metal nanoparticles<\/li>\n<li>Bio-derived nanoparticles such as exosomes with protein and nucleic acid car<\/li>\n<\/ul>\n<p><b>Instrumentation available: <\/b>ICP-MS Perkin Elmer NexION 300d with autosampler,\u00a0Capillary Electrophoresis Beckman Coulter PA 800 plus Pharmaceutical Analysis System,\u00a0HPLC Agilent 1200 series,\u00a0NTA Malvern NanoSight NS500,\u00a0NTA and Zeta Potential meter Particle Metrix ZetaView QUATT,\u00a0DLS Malvern ZetaSizer Nano ZS,\u00a0TGA TD Thermogravimetric analyzer,\u00a0Western Blot and Gel Imager Protein Simple FluorChem E Imager,\u00a0Automated Western Blot Protein Simple WES,\u00a0Freeze dryer (lyophilizer) LABCONCO,\u00a0GPC Malvern Viscotek,\u00a0FPLC AKTA.<\/p>\n<p><strong>Eligible users<\/strong>: Any researcher at 黑料网 or other academic institutions, and industry.<\/p>\n<h2><span id=\"Genetic_Screening\">Genetic Screening<\/span><\/h2>\n<h3><span id=\"黑料网_CRISPR_Core\"><span class=\"largeFont\">黑料网 CRISPR Core<\/span><\/span><\/h3>\n<p><span style=\"font-size: 14px\">The <a href=\"https:\/\/ddi.unc.edu\/crispr\/\">CRISPR Screening Facility<\/a> provides: <\/span><\/p>\n<p><span style=\"font-size: 14px\">Arrayed Screens which includes: Consultation on screen design, benchmarks, and objectives, high quality CRISPR sgRNA arrayed into 96-well or 384-well format, automated assay with in-house robotics, individual acquisition and analysis for each target gene, and deliverable: high quality data sets for individual genes. <\/span><\/p>\n<p><span style=\"font-size: 14px\">Pooled Screens which includes: Consultation on screen design, benchmarks, and objectives, high quality whole mammalian genome CRISPR sgRNA validated pools, assay run by our experienced team, or PI can choose to run assay in their lab, isolation of \u2018hit\u2019 genes, next generation sequencing and bioinformatics, and provides as a deliverable: rank list of genes identified in screen<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>&nbsp; Analytical Services Advanced Translational Pharmacology and Analytical Chemistry (ATPAC) The Advanced Translational Pharmacology and Analytical Chemistry (ATPAC) core lab consists of the Analytical Chemistry and Pharmacology Core (ACPC) lab and the Translational Oncology and Nanoparticle Drug Development Initiative (TONDDI) lab The ATPAC core lab uses analytical chemistry and pharmacologic infrastructure to support the translational &hellip; <a href=\"https:\/\/www.med.unc.edu\/corefacilities\/find-a-core-facility\/drug-development\/\" aria-label=\"Read more about Drug Development\">Read more<\/a><\/p>\n","protected":false},"author":55643,"featured_media":0,"parent":2241,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-5162","page","type-page","status-publish","hentry","odd"],"acf":[],"_links_to":[],"_links_to_target":[],"_links":{"self":[{"href":"https:\/\/www.med.unc.edu\/corefacilities\/wp-json\/wp\/v2\/pages\/5162","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.med.unc.edu\/corefacilities\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.med.unc.edu\/corefacilities\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/corefacilities\/wp-json\/wp\/v2\/users\/55643"}],"replies":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/corefacilities\/wp-json\/wp\/v2\/comments?post=5162"}],"version-history":[{"count":4,"href":"https:\/\/www.med.unc.edu\/corefacilities\/wp-json\/wp\/v2\/pages\/5162\/revisions"}],"predecessor-version":[{"id":5723,"href":"https:\/\/www.med.unc.edu\/corefacilities\/wp-json\/wp\/v2\/pages\/5162\/revisions\/5723"}],"up":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/corefacilities\/wp-json\/wp\/v2\/pages\/2241"}],"wp:attachment":[{"href":"https:\/\/www.med.unc.edu\/corefacilities\/wp-json\/wp\/v2\/media?parent=5162"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}