{"id":5140,"date":"2023-07-02T13:39:11","date_gmt":"2023-07-02T17:39:11","guid":{"rendered":"https:\/\/www.med.unc.edu\/corefacilities\/?page_id=5140"},"modified":"2023-07-02T13:39:46","modified_gmt":"2023-07-02T17:39:46","slug":"vector-production","status":"publish","type":"page","link":"https:\/\/www.med.unc.edu\/corefacilities\/find-a-core-facility\/vector-production\/","title":{"rendered":"Vector Production"},"content":{"rendered":"<div>\n<div class=\"row  oscitas-bootstrap-container\">\n<div id=\"toc_container\" class=\"toc_wrap_left no_bullets\"><p class=\"toc_title\">Contents<\/p><ul class=\"toc_list\"><li><a href=\"#Vector_Production\"><span class=\"toc_number toc_depth_1\">1<\/span> Vector Production<\/a><ul><li><a href=\"#BRAIN_Initiative_Viral_Vector_Core\"><span class=\"toc_number toc_depth_2\">1.1<\/span> BRAIN Initiative Viral Vector Core<\/a><\/li><li><a href=\"#Lenti-shRNA_and_Lenti-cDNA_Core_Facility\"><span class=\"toc_number toc_depth_2\">1.2<\/span> Lenti-shRNA and Lenti-cDNA Core Facility<\/a><\/li><li><a href=\"#黑料网_Vector_Core\"><span class=\"toc_number toc_depth_2\">1.3<\/span> 黑料网 Vector Core<\/a><\/li><\/ul><\/li><\/ul><\/div>\n\n<\/div>\n<\/div>\n<div>\n<h2><span id=\"Vector_Production\"><span style=\"color: #345644\">Vector Production<\/span><\/span><\/h2>\n<h3><span id=\"BRAIN_Initiative_Viral_Vector_Core\">BRAIN Initiative Viral Vector Core<\/span><\/h3>\n<p>The <a href=\"https:\/\/www.unc-neurotools.org\/\">BRAIN Initiative Viral Vector<\/a> provides vectors targeted for neuroscience applications supported by the 黑料网 Neuroscience Center and the NIH BRAIN Initiative.<\/p>\n<h3><span id=\"Lenti-shRNA_and_Lenti-cDNA_Core_Facility\"><span class=\"largeFont\">Lenti-shRNA and Lenti-cDNA Core Facility<\/span><\/span><\/h3>\n<div>\n<p>The <a href=\"http:\/\/www.med.unc.edu\/lenti-shrna\" target=\"_blank\" rel=\"noopener noreferrer\"><span class=\"largeFont\">Lenti-shRNA and Lenti-cDNA Core Facility<\/span><\/a> is premised on three libraries: a human cDNA library in lentiviral vector to over express human genes (under the control of a CMV promoter) and two Open Biosystems\u2019 TRC1 shRNA libraries (to knockdown either mouse or human genes). The shRNA libraries are cataloged as single lentiviral-vector shRNA expression clones directed to ~16,000 human and mouse genes. Each gene is targeted by 4-5 different shRNAs driven by the U6 promoter. Furthermore, control vectors bearing scrambled shRNAs or shRNAs directed against GFP are available. Please note that the shRNAs\u2019 knockdown efficiency has not been validated. The shRNA core provides lentiviral shRNA vectors in the form of glycerol bacterial stocks or vector particle preparations (~10mL). The shRNA core also provides consulting in designing and optimizing shRNA-encoding lentiviral vectors. Upon request, the core can also provide alternate platforms for shRNA delivery, employing a variety of promoters and other cis elements to improve shRNA expression.<\/p>\n<h3><span id=\"黑料网_Vector_Core\"><span class=\"largeFont\">黑料网 Vector Core<\/span><\/span><\/h3>\n<p><a title=\"\" href=\"http:\/\/www.med.unc.edu\/genetherapy\/vectorcore\" target=\"_blank\" rel=\"noopener noreferrer\"><span class=\"largeFont\">黑料网 Vector Core <\/span><\/a> delivers the highest quality AAV vectors (research grade only) to academic, government, foundation, and biotech industry clients.<\/p>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Vector Production BRAIN Initiative Viral Vector Core The BRAIN Initiative Viral Vector provides vectors targeted for neuroscience applications supported by the 黑料网 Neuroscience Center and the NIH BRAIN Initiative. Lenti-shRNA and Lenti-cDNA Core Facility The Lenti-shRNA and Lenti-cDNA Core Facility is premised on three libraries: a human cDNA library in lentiviral vector to over express &hellip; <a href=\"https:\/\/www.med.unc.edu\/corefacilities\/find-a-core-facility\/vector-production\/\" aria-label=\"Read more about Vector Production\">Read more<\/a><\/p>\n","protected":false},"author":55643,"featured_media":0,"parent":2241,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-5140","page","type-page","status-publish","hentry","odd"],"acf":[],"_links_to":[],"_links_to_target":[],"_links":{"self":[{"href":"https:\/\/www.med.unc.edu\/corefacilities\/wp-json\/wp\/v2\/pages\/5140","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.med.unc.edu\/corefacilities\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.med.unc.edu\/corefacilities\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/corefacilities\/wp-json\/wp\/v2\/users\/55643"}],"replies":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/corefacilities\/wp-json\/wp\/v2\/comments?post=5140"}],"version-history":[{"count":1,"href":"https:\/\/www.med.unc.edu\/corefacilities\/wp-json\/wp\/v2\/pages\/5140\/revisions"}],"predecessor-version":[{"id":5141,"href":"https:\/\/www.med.unc.edu\/corefacilities\/wp-json\/wp\/v2\/pages\/5140\/revisions\/5141"}],"up":[{"embeddable":true,"href":"https:\/\/www.med.unc.edu\/corefacilities\/wp-json\/wp\/v2\/pages\/2241"}],"wp:attachment":[{"href":"https:\/\/www.med.unc.edu\/corefacilities\/wp-json\/wp\/v2\/media?parent=5140"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}