{"id":4988,"date":"2019-06-21T13:25:49","date_gmt":"2019-06-21T17:25:49","guid":{"rendered":"https:\/\/www.med.unc.edu\/cgibd\/?page_id=4988"},"modified":"2022-01-10T10:32:48","modified_gmt":"2022-01-10T15:32:48","slug":"rigor-reproducibility","status":"publish","type":"page","link":"https:\/\/www.med.unc.edu\/cgibd\/cores\/advanced-analytics\/rigor-reproducibility\/","title":{"rendered":"Rigor & Reproducibility"},"content":{"rendered":"

Eight steps to Rigorous and Reproducible Experiments in Biomolecular Research at ºÚÁÏÍø:<\/strong><\/p>\n

    \n
  1. If using a core facility,\u00a0consult\u00a0<\/strong>with the core staff in the planning stage. Consult with a statistician if you need help developing a Power Analysis to assure that your results will be adequately powered.<\/li>\n
  2. Design your experiment with\u00a0sufficient controls<\/strong>\u00a0(rigor)\u00a0and replicates <\/strong>(reproducibility).<\/li>\n
  3. Assure that ALL of your reagents (antibodies, cell lines, mice) are\u00a0fully validated <\/strong>(see below).<\/li>\n
  4. Have a clear and\u00a0detailed protoco<\/strong>l (SOP) and data analysis plan. Assure that the protocol is strictly followed or that any deviation is well documented.<\/li>\n
  5. Assure that the staff or students performing the experiment are\u00a0well trained<\/strong>\u00a0and understand each step and the importance of performing them precisely.<\/li>\n
  6. Use only\u00a0well-maintained instrumentation<\/strong>, preferably maintained and operated in a core facility with expert staff (see #1 above).<\/li>\n
  7. Document all steps<\/strong>, reagents, equipment and data analysis methods used in the experiment. Assure that the both the documentation and the data itself are properly stored in a safe data management repository.<\/li>\n
  8. Acknowledge<\/strong> NIH Grant P30 DK034987, the core (by name), and core staff in publications.<\/li>\n<\/ol>\n

     <\/p>\n

    Additional resources: <\/strong><\/p>\n

    Additional Articles about Ab Validation:\u00a0https:\/\/www.euromabnet.com\/guidelines\/articles-about-antibody-validation.php<\/a><\/p>\n

    Learn about the NIH Initiative to Enhance Reproducibility through Rigor and Transparency<\/strong><\/a>. (Video<\/a>)<\/u><\/p>\n

    Resource Authentication Plan<\/strong>:\u00a0https:\/\/grants.nih.gov\/reproducibility\/faqs.htm#V<\/a><\/p>\n

    What Kind of Information Should I Include in My Application\u2019s\u00a0Resource Authentication Plan<\/u><\/strong>?<\/u>\u00a0Check out instructions on\u00a0NIH Nexus Blog.<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

    Eight steps to Rigorous and Reproducible Experiments in Biomolecular Research at ºÚÁÏÍø: If using a core facility,\u00a0consult\u00a0with the core staff in the planning stage. Consult with a statistician if you need help developing a Power Analysis to assure that your results will be adequately powered. Design your experiment with\u00a0sufficient controls\u00a0(rigor)\u00a0and replicates (reproducibility). Assure that ALL … Read more<\/a><\/p>\n","protected":false},"author":78759,"featured_media":0,"parent":2193,"menu_order":21,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-4988","page","type-page","status-publish","hentry","odd"],"acf":[],"yoast_head":"\nRigor & Reproducibility | Center for Gastrointestinal Biology and Disease<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.med.unc.edu\/cgibd\/cores\/advanced-analytics\/rigor-reproducibility\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Rigor & Reproducibility | Center for Gastrointestinal Biology and Disease\" \/>\n<meta property=\"og:description\" content=\"Eight steps to Rigorous and Reproducible Experiments in Biomolecular Research at ºÚÁÏÍø: If using a core facility,\u00a0consult\u00a0with the core staff in the planning stage. 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